The International Tinnitus Journal

The International Tinnitus Journal

Official Journal of the Neurootological and Equilibriometric Society
Official Journal of the Brazil Federal District Otorhinolaryngologist Society

Reach Us Reach Us Whatsapp +44 7367 141882

ISSN: 0946-5448

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Effects of Spirulina on GABA-Receptor Gene Expression in Salicylate-Induced Tinnitus

Author(s): Yin-Ching Chan, Ming-Fu Wang, Juen-Haur Hwang

Objectives: The function of γ-amino butyric acid receptor (GR) was related with tinnitus. But, the effects of Spirulina platensis water extract (SP) on the mRNA expression of GRA β 3 in mice with tinnitus were still unclear.


Method: Eighteen SAMP8 mice were divided into the control group (intraperitoneal injection of saline, once per day), the tinnitus group (intraperitoneal injection of salicylate, 300 mg/kg body weight once per day), and the spirulina group [intraperitoneal injection of salicylate, 300 mg/kg body weight and oral SP supplementation (1000 mg/kg body weight) once per day]. Effects of SP on the mRNA expression of GRA β 3 in the cochlea and brain of mice were studied for 4 days.


Results: Compared to the control group, the tinnitus group had significantly higher tinnitus scores and lower mRNA expression of GRA β 3 gene in the cochlear, brainstem, hippocampus and parahippocampus, temporal lobes, and the frontal lobes. On the other hand, the spirulina group had significantly lower tinnitus scores and higher GRA β 3 gene expression than the tinnitus group in all tested areas.


Conclusion: SP could reduce salicylate-induced tinnitus possibly via increasing the salicylate-induced down-regulation of GRA β 3 gene expression.

Text PDF

Share this  Facebook  Twitter  LinkedIn  Google+