The International Tinnitus Journal

The International Tinnitus Journal

Official Journal of the Neurootological and Equilibriometric Society
Official Journal of the Brazil Federal District Otorhinolaryngologist Society

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ISSN: 0946-5448

Abstract

Effects of Spirulina on GABA-Receptor Gene Expression in Salicylate-Induced Tinnitus

Author(s): Yin-Ching Chan, Ming-Fu Wang, Juen-Haur Hwang

Objectives: The function of γ-amino butyric acid receptor (GR) was related with tinnitus. But, the effects of Spirulina platensis water extract (SP) on the mRNA expression of GRA β 3 in mice with tinnitus were still unclear.


Method: Eighteen SAMP8 mice were divided into the control group (intraperitoneal injection of saline, once per day), the tinnitus group (intraperitoneal injection of salicylate, 300 mg/kg body weight once per day), and the spirulina group [intraperitoneal injection of salicylate, 300 mg/kg body weight and oral SP supplementation (1000 mg/kg body weight) once per day]. Effects of SP on the mRNA expression of GRA β 3 in the cochlea and brain of mice were studied for 4 days.


Results: Compared to the control group, the tinnitus group had significantly higher tinnitus scores and lower mRNA expression of GRA β 3 gene in the cochlear, brainstem, hippocampus and parahippocampus, temporal lobes, and the frontal lobes. On the other hand, the spirulina group had significantly lower tinnitus scores and higher GRA β 3 gene expression than the tinnitus group in all tested areas.


Conclusion: SP could reduce salicylate-induced tinnitus possibly via increasing the salicylate-induced down-regulation of GRA β 3 gene expression.

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